The cardiovascular disorders incidence is mainly linked with some risk factors like lack of physical activity, smoking, overweight, hypertension, stress, hypercholesterolemia, hyperinsulinemia. Diazepam is a medicine of the family benzodiazepine, used to treat various CNS disorders .No study is available for biochemical analysis of diazepam in cardiac dysfunction.. search findings endorse the cardioprotective effect of diazepam in stress-induced cardiac dysfunction in rats. Stress linked disorders are a group of mental disorders induced by stressful life even. Adjustment disorders are mainly triggered by the significant and identifiable change in life which is also known as psychological or physical distress. Diazepam was procured from Sigma Aldrich Chemical. For induction of cardiac dysfunction, Disease Control animals and Disease + Diazepam Treatment animals exposed to daily regular stress for half an hour by forced swimming exercise method for 3 months. Adaptation of heart to exercise training leads to functional, morphological, as well as electrical changes. The forceful exercise creates significant stress on the heart also. Lower ventricular hypertrophy is induced in such a case evident by increased lower ventricular mass, dimension, and wall thickness. chronic stress many times induces stress-mediated myopathy of heart cells known as broken heart syndrome, which is identified by enlargement of the heart. Stress-induced changes may vary depending upon the mode of stress, gender, age, nutrition, race, genetic factors, physical activity, and psychological factors. Diazepam is helpful in maintaining blood pressure and heart rate in coronary artery disease patients. Diazepam shows a cardioprotective effect in stress-induced cardiac remodeled rats. It shows cardioprotection by the restoration of heart size, cardiac marker enzymes, and ionic balance in stress-induced cardiac remodeled rats.