Degradation of lactate dehydrogenase during the cell cycle of burkitts lymphoma cells

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The pattern of lactate dehydrogenase synthesis during all phases of a synchronous cell cycle was determined by labelling the enzyme with [3H]leucine and titrating the amount of LDH present in individual cultures by means of affinity chromatography.

Two different labelling techniques were used, both of which supported the hypothesis of a periodic pattern for total LDH synthesis during the cell cycle, with two peaks of synthesis occurring during S phase, one early in S phase and one late, and two other peaks-ne occurring during the G2/M phase and one during the G1 phase.

When the percentage contribution of each of the individual isoenzymes towards total LDH activity during the cell cycle was taken into account, it became evident that the relative specific incorporation of tritium into both LDH-1 and LDH-5 greatly exceeded that of tritium into LDH-2,3 or 4.

Studies on the relative degradation rates of the total soluble protein, total LDH and the individual LDH isoenzymes gave indications that the isoenzymes were degraded extremely rapidly compared to total soluble proteins.

Aaron Province
Journal of Bioremediation and Biodegradation
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